Origin

Colominic acid, a homopolymer of N-acetylneuraminic acid (NANA) with α (2→8) ketosidic linkages, is produced by special strains of Escherichia coli possessing K1 antigen.※1

Formula

n≒100

CAS No

70431-34-4

Appearance

White amorphous powder.

Purity

More than 98% as NANA (Na salt).

Analytical methods

Resorcinol (Svennerholm) method※2‐※3, and Thiobarbituric acid (TBA) method.※4‐※5 Prior to the determination by TBA method, colominic acid must be hydrolyzed to NANA by neuraminidase.

Molecular weight

Approx. 30,000 Da (DPs ca. 100)

Uses

Substrate for neuraminidase and starting material for preparing sialic acid derivatives.

Storage

Stable for one year or more when stored on desiccated condition at 5 ºC.

References

1. ※1. Y. Uchida, Y. Tsukada and T. Sugimori, Agric.Biol. Chem., 37, 2105 (1973)
2. ※2. J. T. Cassidy, G. W. Jourdian and S. Roseman, J. Biol. Chem., 240, 3501 (1965)
3. ※3. L. Svennerholm, Biochim. Biophys. Acta, 24, 604 (1957)
4. ※4.Y. Uchida, Y. Tsukada and T. Sugimori, J. Biochem., 82, 1425 (1977)
5. ※5. L. Warren, J. Biol. Chem., 234, 1971 (1959)

Package

100 mg, 1 g