Business Fields

UBASTEC-AUTO -Determination of urinary bile acid sulfate(UBAS)-

High specificity toward hepatobiliary diseases

The concentration of urinary bile acid sulfate elevates specifically in hepatobiliary diseases.
Especially, cholestasis and severe liver diseases indicate high value of urinary bile acid sulfate.

UBAS levels in various hepatobiliary diseases※1

USBA levels is corrected by urinary creatinine levels. The concentration of bile acid sulfate in urine is then expressed as μmol/g creatinine.

CIH: Chronic inactive hepatitis CAH: Chronic active hepatitis
Comp. LC: Compensated liver cirrhosis Decomp. LC:Decompensated liver cirrhosis
AH: acute hepatitis EHC: Extrahepatic cholestasis
IHC: Intrahepatic cholestasis  

Determination of urinary bile acid sulfate UBASTEC-AUTO

UBASTEC-AUTO is a simple assay kit for measurement of urinary total bile acid sulfate. UBASTEC-AUTO contains the enzyme, bile acid sulfate sulfatase which acts to the bile acid sulfate specifically.

High detection rate of liver diseases in urine test.

In comparison with the urinary urobilinogen test, detection rate of liver diseases is markedly elevated by UBAS.

Comparison of detection rate of chronic liver diseases.※1

For screening of biliary atresia

UBAS is useful for screening of biliary atresia and for determining whether neonatal jaundice is caused by hepatobiliary diseases or not.

USBA levels in normal infant, neonatal jaundice and biliary atresia※1

Normal neonate value※6

Under 5 μmol/l (without creatinine corrected)

Screening of biliary atresia on neonatal urine.

True negative False positive False negative
Total (1,572 samples) 97.9%
(1,539 samples)
(33 samples)
(0 samples)
mmediately after birth
(975 samples)
(960 samples)
(15 samples)
(0 samples)
1 month of age
(597 samples)
(579 samples)
(18 samples)
(0 samples)

Biliary atresia was not included in total samples.

UBAS levels in neonatal liver diseases※6

Assay principal

Package and Method of storage

Name Constituent reagent Package Method of storage
UBASTEC-AUTO Reagent 1 Enzyme 22 ml use x 2 Store protected
from light
at 4-8 ºC
Reagent 1 Solvent 22 ml x 2
Reagent 2 Enzyme 6 ml use x 2
Reagent 2 Solvent 6 ml x 2
Standard solution 10 ml x 1


  1. ※1. Uenoyama R., Baba S., et al : KAN.TAN.SUI (Japanese), 31, 315-326 (1995)
  2. ※2. Meng G., Mashige F., Kitamura K., et al : Jpn. J. Clin. Chem., 23, 150-157 (1994)
  3. ※3. Adachi K., Kakigawa K., et al : Jpn. J. Clin. Chem., 26, 95-100 (1998)
  4. ※4. Matsui A., et al : The Journal of Pediatrics, 129, 306 (1996)
  5. ※5. Tazuke Y., Kodama N., Adachi K., et al : Jpn. J. Clin. Chem., 21, 249-258 (1992)
  6. ※6. Yamane H., et al : Kobe daigaku igakubu hokengakka kiyo (Japanese), 13, 1-8 (1997)
Pickup Contents
Protected Amino Acids and Peptides
Optically Active (Chiral) Compounds
Organic Synthesis
Custom Synthesis